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Jan 01, 2012018332Dilute to volume with acetone, shake and let stand for 2 min. Pipette 10.00 ml of this extract into another 100 ml volumetric flask. Dilute to volume with acetone and shake. Transfer a portion to the spectrophotometer cell and measure the absorbance at 460 nm. Determine the absorbance of standard glass filter at 465 nm . Calculation.
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